if-m1 media (Biolog Inc)
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if-m1 media
If M1 Media, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/if-m1+media/modified+biolog+if+m1+medium/bio_rxiv__2025__07__13__664342-231-5-12
Average 90 stars, based on 1 article reviews
If M1 Media, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/if-m1+media/modified+biolog+if+m1+medium/bio_rxiv__2025__07__13__664342-231-5-12
Average 90 stars, based on 1 article reviews
if-m1 media - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose"
Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
Journal: bioRxiv
doi: 10.1101/2025.07.13.664342
Figure Legend Snippet: A ) Flt3L bone marrow derived DCs (FLDCs) generated from mice were exposed to 4-hour PFA-killed spores on Biolog M1 Carbon Source Plates with metabolic activity measured with addition of redox dye. B ) Graph displays FLDC redox signal in culture with PFA-killed spores when incubated on each compound, which is normalised to the percentage of activity detected when cultured in α-D-Glucose. Compounds which caused significant metabolic activity of FLDCs compared to negative control are coloured in red. Schematic shows the points in which identified nutrients can feed into glycolysis. C ) FLDCs generated from mice were precultured in basal media prior to addition of spores in the presence of specified nutrients for 6h. D ) Representative flow cytometry overlays display the gMFI of co- stimulatory molecules (CD40 and CD86), CCR7 and MHCII on FL-cDC2s c after exposure to spores whilst individually supplemented with glucose, fructose, dextrin, inosine, glycerol-3-phosphate, mannose or galactose. Graphs display the fold change difference in expression compared to FL-cDC2s not exposed to spores in basal media. B) data from two independent experiments ( n = 5 FLDCs derived from biologically independent animals). D ) data from two independent experiments ( n = 7 FLDCs derived from biologically independent animals). Statistical significance determined by B ) Kruskal Wallis ANOVA with Dunn’s multiple-comparison post-hoc test vs negative control or D ) one-way ANOVA with tukey’s multiple comparison test between indicated groups. *P < 0.05, **P<0.01, ***P < 0.001, **** P<0.0001.
Techniques Used: Derivative Assay, Generated, Activity Assay, Incubation, Cell Culture, Negative Control, Flow Cytometry, Expressing, Comparison
Related Articles
Cell Culture:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Microarray:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Derivative Assay:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Generated:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Activity Assay:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Incubation:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Negative Control:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Flow Cytometry:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Expressing:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Comparison:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Infection:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Titration:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Staining:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Ex Vivo:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Control:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Activation Assay:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. Two Tailed Test:Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose Article Snippet: Following incubation, FLDCs were washed thoroughly in PBS+ 2% FCS + 2μM EDTA (FACS buffer, all sigma) for onward analysis. |
